Cytotoxicity of cleaning agents for ocular prostheses.

BACKGROUND: Polymethylmethacrylate (PMMA) is the most used material for the manufacturing of eye prostheses. OBJECTIVES: To investigate the cytotoxicity of different cleaning agents for ocular prostheses on human conjunctival cells. MATERIAL AND METHODS: Six groups of specimens were created (saline, soap, 4% chlorhexidine, hydrogen peroxide, 1% triclosan, and citronella oil). Three specimens were made for each disinfectant at each disinfection period (1, 7, 15, 30, 60, and 90 days), totaling 108 specimens. Thus, the specimens were disinfected, with different disinfectants, for different periods of time. After each disinfection process, the specimens were washed with sterile distilled water. A human conjunctival cell line was grown on the acrylic resin specimens and then cytotoxicity tests (MTT and Neutral Red (NR)) were performed. A negative control (untreated cell cultures) and positive control (Tween 20) were created. Two-way analysis of variance (ANOVA) and Bonferroni test were performed (p < 0.05). RESULTS: For the MTT and NR tests, when there was a significant difference between the disinfectant and negative control, the disinfectant generated a significant reduction in cell proliferation most of the time. CONCLUSIONS: All reductions in cell proliferation caused by the disinfectants were clinically acceptable. All disinfectants tested in this study were found to be non-cytotoxic to human conjunctival cells.

Treatment of Masticatory Muscle Pain with Acupuncture: Is It Necessary to Associate with Occlusal Splints?

Background: Temporomandibular disorders (TMDs) are treated by different modalities including splints, physiotherapy, and acupuncture. Although all of these offer evidencebased benefits to the patients, avoiding overtreatment is of paramount importance. Objectives: To assess the effectiveness of muscle pain treatment with acupuncture combined with or without occlusal splints. Methods: Recruited patients were allocated to G1 (acupuncture) and G2 (acupuncture and occlusal splint) groups and treated in four consecutive weekly sessions (P1, P2, P3, and P4). The reported pain (RP) and the pressure pain threshold (PPT) of the masseter and temporalis muscles were assessed before and after each session. Results: RP decreased after each session in G1, except at P4. In G2, the RP decreased only after the first session, and the PPT did not vary. Conclusion: The assessed treatments did not influence the PPT levels of the masseter and anterior temporalis muscles in patients with temporomandibular disorders. Our findings suggest that occlusal splints may not be mandatory along with the acupuncture treatment.

Efeitos da reabilitação oral com novas próteses totais convencionais sobre a força de mordida e a amplitude eletromiográfica dos músculos supra-hióideos e esternocleidomastóideos e suas correlações com a dimensão vertical de oclusão / Effects of oral rehabilitation with new conventional complete dentures on bite force and electromyographic amplitude of suprahyoid and sternocleidomastoid muscles and their correlation with vertical occlusal dimension

Objetivos: Analisar os efeitos da reabilitação oral com próteses totais sobre o força máxima de mordida e eletromiografia dos músculos supra-hióideos e esternocleidomastóideo e suas correlações com a DVO. Métodos: Pacientes usuários de próteses totais insatisfatórias participaram em três sessões (T0, T1 e T2). No T0, enquanto os pacientes ainda usavam suas próteses velhas, eles foram submetidos a exames de força de mordida e eletromiografia dos músculos supra-hióideos e esternocleidomastóideos. Novamente, esses exames foram repetidos e a DVO foi medida enquanto os pacientes usavam as suas próteses novas e as velhas, 30 dias após a instalação das novas próteses (T1). Cem dias após a instalação das novas próteses (T2) os exames foram repetidos. Os dados foram submetidos aos testes de normalidade de ShapiroWilk, análise de variância (ANOVA), correlação de Pearson e regressão linear, todos com 5% de significância. Resultados: Quinze pacientes participaram do estudo. Nenhuma diferença estatisticamente significante foi observada para força de mordida e eletromiografia. Porém, os testes de correlação e regressão demonstraram importantes interações entre DVO e força de mordida, e DVO e eletromiografia durante a deglutição para os músculos supra-hióideos. Conclusão: A reabilitação não impactou a força de mordida nem as atividades dos músculos estudados (eletromiografia). Por outro lado, a DVO demonstrou ser um fator importante para força de mordida e deglutição de água após a reabilitação(AU)

Objectives: The purpose of this study was to assess the effects of oral rehabilitation with complete dentures on bite force and electromyography of the suprahyoid and sternocleidomastoid muscles, and their correlation with OVD. Materials and Methods: Patients wearers of unsatisfactory removable complete dentures were attended in three sessions (T0, T1 and T2). At T0, while the patients still wore the old dentures, they were submitted to bite force and surface electromyographic exams of the suprahyoid and sternocleidomastoid muscles. These exams were repeated and the OVD was measured while the patients wore their old and new prostheses, 30 days after insertion of the new prosthesis (T1). The exams were repeated 100 days after the insertion of the new prosthesis (T2). The data were submitted to the ShapiroWilk normality test, analysis of variance (ANOVA), and Pearson correlation and linear regression, all with 5% significance. Results: Fifteen patients participated in the study. No statistically significant difference was observed for bite force or electromyography in T0, T1, or T2. However, the correlation and regression tests showed important interactions between the OVD and bite force, as well as the OVD and electromyography during deglutition for the suprahyoid muscles. Conclusion: Rehabilitation did not impact bite force nor the activity of the assessed muscles (electromyography). On the other hand, OVD was shown to be an important factor for bite force, and deglutition of water after rehabilitation(AU)

The Impact of Surgery and Radiotherapy on Health-Related Quality of Life of Individuals with Oral and Oropharyngeal Carcinoma and Short-Term Follow up after Treatment.

OBJECTIVE: This study aimed to evaluate the quality of life of patients with oral or oropharyngeal cancer by using specific questionnaires (QLQ-C30 and QLQ-HandN35), varying according to the location of the tumor (oral cavity or oropharynx) and the treatment performed (only surgery or surgery associated with radiotherapy). METHODS: Fifty patients were enrolled in this study and answered the EORTC QLQ-C30 and EORTC HandN35 questionnaires, before (baseline), at 1 week, and 3 months after treatment. Internal consistency reliability was calculated with the Cronbach coefficient. The Kruskal-Wallis and Wilcoxon tests were applied and P.

Diagnóstico diferencial de limitação de abertura bucal / Differential diagnosis of limited mouth opening

O objetivo do presente estudo é apresentar uma revisão de literatura exemplificando alguns dos possíveis fatores etiológicos de limitação de abertura bucal e ilustrar o caso de um paciente diagnosticado com hiperplasia do processo coronoide. Foi realizada uma busca nas bases de dados PUBMED/MEDLINE, Scielo, Bireme e Google Acadêmico utilizando-se inicialmente o termo "limited mouth opening" e "limitação de abertura bucal" no mês de novembro de 2016. Além disso, foi realizada busca manual em periódicos e nas bases de dados com novas palavras-chave como disfunção temporomandibular, tétano, traumas, doenças genéticas e imitação de abertura bucal e hiperplasia do processo coronóide. Foram incluídos estudos clínicos, revisões de literatura, revisões sistemáticas e relatos de caso publicados em idioma inglês ou português. Foram descritos os aspectos relevantes sobre desordens na articulação temporomandibular, tétano, traumas e fraturas, lesões orais, neoplasias e efeito colateral de radio e quimioterapia, desordens genéticas, hiperplasia do processo coronóide. O diagnóstico da limitação de abertura bucal é complexo devido ao grande número de fatores que podem provocar o problema. Diante disso, é de suma importância que seja realizada correta anamnese e investigação minuciosa do histórico do paciente, além da realização de exames complementares, permitindo assim, que a causa do problema seja detectada e que o tratamento correto seja aplicado, por meio de abordagem multidisciplinar, melhorando a qualidade de vida e devolvendo saúde ao paciente(AU)

The objective of this study is to present a review of literature exemplifying some of the possible etiological factors of oral opening limitation and to illustrate the case of a patient diagnosed with coronoid hyperplasia. A searched was made on PUBMED / MEDLINE, Scielo, Bireme and Google Scholar databases initially using the term "limited mouth opening" and "limitação de abertura bucal" in the month of November 2016. In addition, a manual search was carried out in journals and with new keywords like temporomanbidular disorders, tetanus, traumas, genetic disorders and limited open mouth and coronoid process hiperplasia. Clinical studies, literature reviews, systematic reviews and case reports published in English or Portuguese were included in the review. Relevant aspects of temporomandibular joint disorders, tetanus, trauma and fractures, oral lesions, neoplasys and side effects of radiotherapy and chemotherapy, genetic disorders, and coronoid process hyperplasia were described. The diagnosis of oral opening limitation is complex because of the many causes of the problem. Therefore, it is very important that a correct anamnesis and detailed investigation of the patient's history be performed, in addition to performing complementary tests, thus allowing the cause of the problem to be detected and treated correctly, through a multidisciplinary approach, improving the quality of life and healing the patient(AU)

Efeito da reabilitação oral com próteses totais convencionais sobre os estímulos perceptivos e a amplitude eletromiográfica do músculo orbicular da boca / Effect of oral rehabilitation with conventional complete dentures on stimulus perception and the electromyographic amplitude of orbicularis oris muscle

O envelhecimento acarreta uma série de modificações funcionais no corpo humano, incluindo o sistema estomatognático. Grande parcela de idosos sofre perdas dentárias ao longo da vida e necessita de tratamento protético para devolução da função mastigatória. Muitos optam pelo uso de próteses totais convencionais, que podem causar alguns desconfortos orais e modificações na amplitude eletromiográfica do músculo orbiculares da boca, cuja avaliação antes e após tratamento reabilitador justifica-se para o conhecimento da função muscular dos pacientes em situações orais diferentes. O objetivo deste estudo foi avaliar o efeito da reabilitação oral com novas próteses totais convencionais sobre os estímulos perceptivos e a amplitude eletromiográfica do músculo orbicular da boca. Foram selecionados pacientes da Clínica de Prótese Total da Faculdade de Odontologia de Araçatuba (UNESP) e da Clínica do Curso de Especialização em Prótese da Associação Paulista de Cirurgiões Dentistas (APCD) - Araçatuba, de acordo com critérios de inclusão pré-estabelecidos. Todos os pacientes selecionados assinaram um Termo de Consentimento Livre e Esclarecido. Esse estudo foi aprovado pelo Comitê de Ética em Pesquisa da Faculdade de Odontologia de Araçatuba - UNESP. Antes da instalação das novas próteses (T0), todos os pacientes, usando suas próteses velhas, responderam ao questionário de Percepção e foram submetidos a exames eletromiográficos de superfície do músculo orbicular da boca durante repouso, sucção de água com canudo e pronúncia das sílabas Ba, Ma, Pa e da palavra Mississipi. Os procedimentos acima foram repetidos após 30 (T1) e 100 dias (T2) da instalação das novas próteses totais. Os dados obtidos pelo questionário de Percepção foram submetidos ao teste Teste Q de Cochran com 5% de significância (p<0,05). Todos os dados obtidos por meio dos exames eletromiográficos foram submetidos ao teste de Análise de Variância (ANOVA) com medidas repetidas com dois fatores de variância e posteriormente, foram submetidos ao teste de Tukey com 5% de significância (p<0,05). Quinze pacientes, com média de 65 anos de idade, participaram deste estudo. Para o questionário de Percepção, houve diferença significativa para a sensação de desconforto no período T2. Houve redução da amplitude eletromiográfica, entre os períodos de análise, durante o repouso e sucção e a amplitude eletromiográfica foi maior no fascículo inferior durante a fonética. Pôde-se concluir os efeitos da reabilitação oral com novas próteses totais convencionais foram: redução da sensação de desconforto oral aos 100 dias após a instalação das próteses; Redução da amplitude eletromiográfica no fascículo superior do músculo orbicular da boca, durante o repouso; Aumento da amplitude eletromiográfica aos 30 dias, seguido de diminuição aos 100 dias no fascículo superior, e diminuição aos 100 dias no fascículo superior, durante a sucção; Diminuição da amplitude eletromiográfica aos 30 dias, com diferença estatística, exceto para a sílaba Pa, no fascículo inferior, durante os testes fonéticos. Além disso, o fascículo inferior é mais solicitado que o superior durante o repouso e maioria atividades funcionais(AU)

Aging causes several modifications throughout the human body, including the stomatognathic system. A large portion of elderly suffers teeth loss along life and needs prosthetic treatment for devolution of the masticatory function. Many individuals opt for conventional complete dentures that might cause some oral discomfort and modification of orbicularis oris muscle's electromyographic amplitude which evaluation before and after rehabilitation treatment is justified by the knowledge of muscle function of the individuals in different oral patterns. This study aimed to evaluate the effect of oral rehabilitation with conventional complete dentures on stimulus perception and the electromyographic amplitude of the orbicularis oris muscle. Patients from Complete Denture Clinic of Araçatuba Dental School and from the Clinic of Prosthesis Specialization Course of São Paulo Dental Surgeon Association- Araçatuba, were selected according to the previously established inclusion criteria. The selected patients signed a Consent Form. This study was approved by the Ethic Committee of Araçatuba Dental School. Before the new prostheses installation (T0), all the patients answered the Perception questionnaire and were submitted to surface electromyographic exams of orbicularis oris muscle during rest, suction of water with a straw and pronunciation of syllabus Bah, Mah Pah and the word Mississippi, wearing their old prosthesis. The procedures above were repeated 30 (T1) and 100 days (T2) after the new prostheses installation. The data obtained from the Perception questionnaire were submitted to Q Cochran Test with 5% significance (p<0.05). All data obtained from the electromyographic exams were submitted to Variance Analysis test (ANOVA) with repeated measures with two variance factors and posteriorly, the data of exams were submitted to the Tukey test with 5% significance (p<0.05). Fifteen patients, with mean age of 65 years, agreed to participate in this study. In relation to the Perception questionnaire, there was a statistical difference in oral discomfort in T2. There was reduction of electromyographic amplitude, among analysis periods, during rest and suction and the electromyographic amplitude was higher on lower fascicle during phonetic. It was concluded that the effects of rehabilitation with new conventional complete dentures on stimulus perception were: Decrease of oral discomfort on 100th day after the prostheses installation; Decrease of electromyographic amplitude on upper fascicle of orbicularis oris muscle, during rest; Increase of electromyographic amplitude of upper fascicle on 30th day followed by its decrease on 100th day, during suction. Decrease on electromyographic amplitude on 30th day, with statistical difference, except to syllable Pa, on lower fascicle, during phonetic tests. In addition, the lower fascicle is more required than upper one during rest and most of functional activities(AU)